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| 職 稱 |
副教授 |
| 出生年 |
1964 |
| 最高學歷 |
加拿大 Saskachewan 大學博士 |
| 專 長 |
植物分子生物, 植物生長與發育, 植物分子逆境反應 |
| E-mail |
kewu@ntu.edu.tw |
| 研究室 |
生命科學館 1026 室 |
| 電話 |
3366-4546 |
| 傳真 |
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1) 組蛋白修飾對植物基因表達的影響
2) ERF蛋白在植物生長發育與植物逆境反應的作用 |
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表觀遺傳學 （Epigenetics）是指由於非基因序列改變所引起的可遺傳的基因表達的變化。表觀遺傳的分子機理包括DNA的甲基化，組蛋白的修飾以及染色質的重塑
（Chromatin remodeling）等。最近的研究表明，
RNA 特別是非編碼 (noncoding) RNA
在表觀遺傳現象中也扮演重要的角色。植物生長與發育過程中的基因表達與染色質的結構的變化密切相關，而作為染色質的重要組成部分組蛋白上的蛋白質修飾在基因表達的調控上起重要的作用。組蛋白質修飾包括磷酸化﹐甲基化﹐
乙醯化
(acetylation)
以及
ubiquitination
等。一般而言﹐若染色質的組蛋白乙醯化的程度較高,
則該部位的結構較為鬆散, 有利於基因的表達。反之,
若染色質的組蛋白乙醯化的程度較低,
則該部位的結構較為緻密, 不利於基因的表達。我們的研究發現,
組蛋白乙醯化與脫乙醯化參與了植物激素的信號傳導以及植物對逆境的反應。我們正在進一步研究組蛋白修飾對植物基因表達的影響以及在植物生長與發育的作用。這些研究將揭示植物生長與發育以及植物逆境反應過程中訊息傳導的分子機理。
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阿拉伯芥是一種廣泛應用于分子生物學研究的模式植物。 阿拉伯芥的開花可以受到光周期調節。
圖中顯示野生型 (A, B) 以及突變體 (C, D) 阿拉伯 芥生長在長日照 (A, C) 或者短日照 (B, D) 條件下 的不 同開花時間。
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本實驗室為植科所新成立的研究室, 誠摯歡迎對研究工作有熱誠的年輕人加入我 們的研究行列。
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Wu
K, Zhang L, Zhou C ,Yu CW, and Chaikam V. (2008) HDA6 is required for jasmonate response, senescence and flowering in Arabidopsis. J. Exp. Bot. 59: 225–234.
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Fu W., Wu K. and Duan J. (2007) Sequence and expression analysis of histone deacetylases in rice. Biochem. Biophys. Res. Commun. 356：843-850.
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Sridha S. and Wu K. (2006)
Identification of AtHD2C
as a novel
regulator of abscisic
acid responses in
Arabidopsis. Plant J.
46:
124-133.
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Zhou C., Zhang L., Duan J., Miki B. and
Wu K. (2005)
HDA19 is involved in
jasmonic acid and ethylene signaling of pathogen-response
in Arabidopsis. Plant Cell
17: 1196–1204.
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Yang Z., Tian L., Latoszek-Green M., Brown D. and
Wu K. (2005)
Arabidopsis ERF4 is a transcriptional repressor capable
of modulating ethylene and abscisic acid responses. Plant Mol. Biol.
58:585-596.
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Yan Y., Lin X., Zhang Y., Wang L., Li
Y., Wu K. and Huang S. (2005)
Isolation of peanut cDNAs encoding arachins and conglutins by expressed
sequence tags. Plant Sci.
169: 439-445.
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Tian L., Wu
K., Hannam C., Latoszek-Green M., Sibbald S., Hu M., Brown
D. and
Miki B. (2005) Analysis of eIF4A
promoter elements for expression in plants. J. Plant Physiol. 162:
1355-1366.
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Zhou C., Labbe H.,
Sridha S.,
Wang L., Tian L.,
Latoszek-Green M., Yang Z., Brown D., Miki
B., and Wu K. (2004) Expression and
function of HD2-type histone deacetylases in Arabidopsis development. Plant J. 38:
715-724.
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Zhou C., Miki B., and
Wu K. (2003)
CHB2,
a member of the SWI3 gene family, is a global regulator in
Arabidopsis. Plant Mol.
Biol. 52: 1125-1134.
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Wu K, Tian L., Zhou C., Brown D., and Miki B. (2003) Repression of gene expression by
Arabidopsis HD2 histone deacetylases. Plant J. 34: 241-247.
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Wu K., Hu M., Martin T.,
Wang C., Li X., and Miki B. (2003) The cryptic enhancer elements of the
tCUP promoter. Plant Mol. Biol. 51: 351-362.
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Tian L., Wu
K., Ouellet T., Foster E.,
Latoszek-Green M., Sibbald S., Miki B., and Brown D. (2003) Activity of
elements from the tobacco cryptic promoter,
tcup,
in conifer tissues. In Vitro Cell Devel Biol - Plant 39:193-202.
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Wu K., Tian L.,
Hollingsworth J., Brown D. and Miki B. (2002) Functional analysis of
tomato
Pti4 gene in Arabidopsis. Plant Physiol. 128: 30-37.
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Wu K., Hu M.,
Martin T., Wang C., Li X., and Miki B. (2002) The cryptic enhancer elements of
the tCUP promoter. Plant Mol. Biol. 51: 351-362.
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Tian L., Wang, H.,
Wu K., Latoszek-Green
M., Hu M., Miki B., and Brown D. (2002) Efficient recovery of transgenic plants
through organogenesis and embryogenesis using a cryptic promoterto drive marker
gene expression. Plant Cell Rep. 20:1181-1187.
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Malik K., Wu,
K., Li, X., Martin-Heller, T., Hu, M., Foster, L., Tian L.,
Jordan
, M.,
Brown, D., Gleddie, S., Simmonds, D., Zheng, S., Simmonds, J., and Miki, B.
(2002)
A
constitutive gene expression system derived from the
tCUP cryptic gene
regulatory elements.
Theor. Appl. Genet. 105: 505-514.
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Miki B., Brown D., Tian L., Malik K., and
Wu
K. (2002) The role of histone deacetylases in flowering. Flowering Newsletter 33: 3-6.
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Akache B., Wu
K. and Turcotte B. (2001) Phenotypic analysis of genes encoding yeast zinc
cluster proteins.
Nucleic Acids Res.
29: 2181-2190.
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Wu K., Malik K.,
Tian L., Hu M., Martin T., Foster L., Brown D. and Miki B. (2001) Enhancers and
core promoter elements are essential for the activity of a cryptic gene
activation sequence from tobacco,
tCUP. Mol. Genet. Genomics 265:
763-770.
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Wu K., Tian L.,
Malik K., Brown D. and Miki B. (2000) Functional analysis of HD2 histone
deacetylase homologs in Arabidopsis
thaliana. Plant J. 20: 19-28.
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Wu K., Malik K.,
Tian L., Brown D. and Miki B. (2000) Functional analysis of a RPD3 histone
deacetylase homolog in Arabidopsis
thaliana. Plant Mol. Biol. 44: 167-176.
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生物技術核心實驗 |
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植物生理學 |
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植物分子生物學研究方法 |
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植物科學專題研究討論 |
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