國立台灣大學 | 生命科學院
 
>首頁 >師資 >張英峯 副教授 (Ing-Feng Chang, Associate Professor)
張英峯 副教授 (Ing-Feng Chang, Associate Professor)
 
職 稱 副教授
出生年 1970
最高學歷 美國加州大學河邊分校博士
專 長 植物蛋白質體學,植物生物化學
E-mail ifchang@ntu.edu.tw
研究室 R905
電話 02-33662534
傳真  
近年研究主題
第一方向- 14-3-3蛋白 之結合蛋白與其生理功能。
第二方向- CDPK基質磷酸化之生理意義 。
研究室簡介
    我於美國加州大學河邊分校取得博士學位期間研習阿拉伯芥核醣體(ribosome)蛋白質體,之後於美國內華達大學雷諾分校從事博士後研究繼續研習阿拉伯芥之磷酸化蛋白質。我的專長是植物蛋白質體學及植物生物化學。本實驗室為新成立的研究室,竭誠歡迎加入我們的研究團隊。本實驗室專研磷酸化蛋白質,近期研究方向可分兩方面進行:

第一方向- 14-3-3蛋白之結合蛋白與其生理功能 14-3-3蛋白是鷹架蛋白(scaffold protein),在人類被發現與疾病有關(例如癌症及阿茲海默症)。14-3-3蛋白會和磷酸化蛋白(phosphorylated protein)結合,結合蛋白通常有Mode I (K/R XX S/T X P) 或Mode II (K/R XXX S/T X P) binding motif。前人研究發現在阿拉伯芥(Arabidopsis)植物體內有12個會表現的14-3-3 蛋白,這些蛋白參與逆境(stress)反應的調控,然而詳細機轉未明。利用蛋白質體學(proteomics) 配合雙重性親合純化(tandem affinity purification)及二維液體層析質譜學(2D-LC mass spectrometry)為技術,超過一百個阿拉伯芥蛋白被發現與其結合。這些結合蛋白包括轉錄因子(transcription factor)、轉譯因子、膜蛋白受體(membrane receptor)等重要蛋白。本實驗室擬利用相同之系統生物學(system biology)技術發掘更多結合蛋白,同時鑽研14-3-3蛋白及其結合蛋白在逆境調控之生理意義。

第二方向- CDPK基質磷酸化之生理意義 Calcium-dependent protein kinase(CDPK)是植物鈣離子訊息之解碼者(calcium-sensor),在阿拉伯芥植物之基因組裡有三十四個CDPK基因及八個類CDPK基因CRK。前人利用質譜儀已鑑定出數個基質磷酸化位點(phosphorylation site)。本實驗室擬深入探討基質磷酸化之生理意義及其調控機制。

代表著作

(A)期刊論文

  1. Singh, S.K., Chien, C.T., Chang, I,.F.* 2016. The Arabidopsis glutamate receptor-like gene GLR3.6 controls root development by repressing the Kip-related protein gene KRP4. Journal of Experimental Botany 67(6):1853-69. (SCI)
  2. Wu, T.Y.,Kao, P., Chang, C.L., Hsu, P.H., Chou, C.H., Chang, I,.F.* 2015. Phosphoproteomic profiling of microsomal fractions in leaves of Cogon grass (Imperata cylindrica). Plant OMICS (SCI)
  3. Lee, T.C., Xiong, W., Paddock, T., Carrieri, D., Chang, I.F., Chiu, H.F., Ungerer, J., Juo, S.H., Maness, P.C., Yu, J. 2015. Engineered xylose utilization enhances bio-products productivity in the cyanobacterium Synechocystis sp. PCC 6803. Metabolic Engineering 30: 179-189. (SCI)
  4. Chen, Y.T., Shen, C.H., Lin, W.D., Chu, H.A., Huang, B.L., Kuo, C.I., Yeh, K.W., Huang, L.C., Chang, I.F. 2013. Small RNAs of Sequoia sempervirens during rejuvenation and phase change. Plant Biology 15: 27-36. (SCI)
  5. Huang, S.J., Chang, C.L., Wang, P.H., Tsai, M.C., Hsu, P.H., Chang, I.F.* 2013. A type III ACC synthase, ACS7, is involved in root gravitropism in Arabidopsis thaliana. Journal of Experimental Botany 64: 4343-4360. (SCI)
  6. Chen, Y.T., Shen, C.H., Lin, W.D., Chu, H.A., Huang, B.L., Kuo, C.I., Yeh, K.W., Huang, L.C., Chang, I.F. 2013. Small RNAs of Sequoia sempervirens during rejuvenation and phase change. Plant Biology 15: 27-36. (SCI)
  7. Chu, H.A., Chang, I.F., Shen, C.H., Chen, Y.T., Wang, H.T., Huang, L.C., Yeh, K.W. 2012. Photosynthetic properties and photosystem stoichiometry of in vitro-grown juvenile, adult, and rejuvenated Sequoia sempervirens (D. Don) Endl. Botanical Studies53: 223-227. (SCI)
  8. Chang, I.F.*, Hsu, J.L., Hsu, P.H., Sheng, W.A., Lai, S.J., Lee, C., Chen, C.W., Hsu, J.C., Wang, S.Y., Wang, L.Y., Chen, C.C. 2012. Comparative phosphoproteomic analysis of microsomal fractions of Arabidopsis thaliana and Oryza sativa subjected to high salinity. Plant Science 185-186: 131-142. (SCI)
  9. Curran, A.+, Chang, I.F.+, Chang, C.L. Garg, S., Garg, S., Miguel, R.M., Barron, Y.D., Li, Y., Romanowsky, S., Cushman, J.C., Gribskov, M., Harmon, A.C., Harper, J.F.* 2011. Calcium-dependent protein kinases from Arabidopsis show substrate specificity differences in an analysis of 103 substrates. Frunt Plant Science 2:36 (+: equal contributions) (SCI)
  10. Chang, I. F., Chen, P. J., Shen, C. H., Hsieh, T. J., Hsu, Y. W., Huang, B. L., Kuo, C. I., Chen, Y. T., Chu, H. A., Yeh, K. W., Huang, L. C.* 2010. Proteomic profiling of proteins associated with the rejuvenation of Sequoia sempervirens (D. Don) Endl. Proteome Science 8: 64. (SCI)
  11. Hsu, J. L., Wang, L. Y., Wang, S. Y., Lin, C. H., Ho, K. C., Shi, F. K. and Chang, I. F.* 2009. Functional phosphoproteomic profiling of phosphorylation sites in membrane fractions of salt-stressed Arabidopsis thaliana. Proteome Science 7: 42. (SCI)
  12. Chang,I. F.+*, Curran A.+, Woolsey R., Quilici D., Cushman J., Mittler R., Harmon A., Harper J. F.* 2009. Proteomic profiling of tandem affinity purified 14-3-3 protein complexes in Arabidopsis thaliana. Proteomics 9: 2697-2985. (+: equal contributions) (SCI)
  13. Hsu, Y. W., Sihgh, S. K., Chiang, M. Y., Wu, Y. Y., Chang, I. F. 2009.Strategies to lower greenhouse gas level by rice agriculture. African Journal of Biotechnology 8: 126-132.
  14. Chang, I. F. 2008. Ecotypic variation of a medicinal plant Imperata cylindrica populations in Taiwan: mass spectrometry-based proteomic evidence. Journal of Medicinal Plants Research 2: 71-76.
  15. Hsiao, H. Y., Chang, I. F. 2008. Proteomic profiling of rat brain discharged by ultrasound associated with high frequency electro-magnetic field. Proteomics Research Journal 1: 41-53.
  16. Chang, I. -F. 2006. Mass-spectrometry based proteomic analysis of the epitope-tag affinity purified protein complex in eukaryotes.  Proteomics  6: 6158-6166 (Review). (SCI)
  17. Chang, I. -F. and Chou, C. H. 2006. Ecotypic variation of Imperata cylindrica populations in Taiwan: II. Physiological and biochemical evidences.  Botanical Studies  47: 175-184. (SCI)
  18. Rodriguez Milla, M. A., Uno, Y., Chang, I. –F., Townsend, J., Maher, E. A., Quilici, D. and Cushman J. C. 2006. Arabidopsis AtCPK11, a calcium-dependent protein kinase, phosphorylates AtDi19, a nuclear zinc finger protein.  FEBS Letters  580: 904-911. (SCI)
  19. Rodriguez Milla, M. A., Townsend, J.,  Chang, I. –F.  and Cushman, J. C. 2006. Arabidopsis Di19-Related Genes Encode a Novel Family of Proteins With Two Unusual Cys2/His2 Zinc-Finger Motifs Evolutionary Conserved Involved in ABA-Independent Stress-Signaling Pathways.  Plant Molecular Biology  61: 13-30. (SCI)
  20. Chang, I. –F. , Hsiao, H. Y. 2005. Induction of RhoGAP and pathological changes characteristic of Alzheimer’s disease by UAHFEMF discharge in rat brain. Current Alzheimer Research  2:559-569. (SCI)
  21. Zanetti, M. E., Chang, I. -F., Galbraith, D. W., Bailey-Serres, J. 2005. Immunopurification of polyribosomal complexes of Arabidopsis for global analysis of gene expression.  Plant Physiology  138: 624-635. (SCI)
  22. Chang, I. -F.*, Szick-Miranda*, K., Pan, S., Bailey-Serres, J. 2005. (*: equal contributions) Proteomic Characterization of Evolutionarily Conserved and Variable Proteins of Arabidopsis Cytosolic Ribosomes.  Plant Physiology  137: 848-862. (SCI)
  23. Yang, C. M., Wang, M. C., Lu, Y. F., Chang, I. -F., Chou, C. H. 2004. Humic substances affect the activity of chlorophyllase.  Journal of Chemical Ecology  30: 1057-1065. (SCI)
  24. Yang, C. M., Chou, C. H.,  Chang, I. -F. and Lin, S. J. 2004. Effects of three allelopathic phenolics on chlorophyll accumulation of rice (Oryza sativa) seedlings: II Stimulation of consumption-orientation.  Bot. Bull. Acad. Sin.  45: 119-125. (SCI)
  25. Williams, A.*, Werner-Fraczek. J.*,  Chang, I.-F.,* Bailey-Serres, J. (*: equal contributions) 2003. Regulated phosphorylation of 40S ribosomal S6 in root tips of maize. Plant Physiology  132: 2086-2097. (SCI)
  26. Barakat, A., Szick-Miranda, K.,  Chang, I. -F., Guyot, R., Blanc, G., Cooke, R., Delseny, M., Bailey-Serres, J. 2001. The organization of cytoplasmic ribosomal protein genes in the Arabidopsis genome.  Plant Physiology  127: 398-415. (SCI) .
(B)著作

Kao, P., Wu, T. Y., Chang, C. L., Chou, C. H., Chang, I. F. 2011. Decreasing of Population Size of Imperata cylindrica Mangrove Ecotype & Sea-Level Rising, Global Warming Impacts - Case Studies on the Economy, Human Health, and on Urban and Natural Environments, Stefano Casalegno (Ed.), ISBN: 978-953-307-785-7, InTech.

 

Lab Members
Ming-Jun Chen (陳明峻)  Part-time Research Assistant
Chia-Lun Chang (張家綸)  Master student
Ching-Huang Lin (林靖凰)  Master student
Shiuan-Jeng Lai (賴軒正)  Master student
Wei-An Sheng (盛維安)  Master student
Shu-Ying Wang (王舒瑩)  Master student
Ya-Wen Hsu (許雅雯)  Ph.D. student
Shashi Kant Singh (夏許)  Ph.D. student
開設課程
 普通植物學  
 蛋白質體學  
 植物生理學  
   
   
   
   
   
 
Copyright © 2004, 國立台灣大學植物科學研究所

最後更新: