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San-San Tsay, Professor
Position Professor
Year of Birth 1943
Highest Degree Ph. D, Oklahoma State University. USA 1971
Specialty bacterial physiology, applied microbiology, environmental microbiology
Laboratory Life Science Building Rm1129
Telephone 02-33662535
02-23630231 ext.2134
Current Research Interests : (Microbiology Lab) 

1. Investigate and identify the microorganisms in hot springs of geothermophilic area of Taiwan.
2. Application potential of the thermophilic isolates.

Present progress:
From 1998 summer, our lab started to investigate the microorganisms in hot springs of Taiwan. Over 280 thermophilic and thermotolerant fungi which could grow above 55 ℃ were isolated. Part of them had been identified as followed: Aspergillus fumigatus, Humicola insolens, Penicillium dupontii, Thermomyces lanuginosus and Rhizoctonia sp. Strains of T. lanuginosus can grow over 60℃ even reach to 64℃. It may be the maximum growth temperature for fungi so far recorded. Over 1000 aerobic thermophilic bacterial had also been isolated, purified and preserved. Only part of the isolates had been analyzed by morphological, physiological, biochemical, genetic and molecular biological methods. They were identified as Bacillus, Thermus, Meiothermus, Pseudoxanthomonas, Deinococcus and Rubrobacter et al. Besides, we found some of the isolates are quite different from the type strains already reported and we had classified these indigenous thermophile as new species. They are Meiothermus taiwanensis (ref 4), Pseudoxanthomonas taiwanensis (ref 3) and Rubrobacter taiwanensis (ref 2). The thermostable enzymes and antibiotic producing abilities as well as radiation resistance activities of these isolates had also been screened. The enzymes had been tested including amylases, proteases, lipases and superoxide dimutase (SOD). Amoung these isolates, we found several potent enzyme producing species, such as Brevibacillus thermoruber could produce thermostable Lon protease (ref 1), Geobacillus sp. NTU-249 produced thermostable metalloprotease, Pseudoxanthomonas taiwanensis CB-226T (ref 3) contain denitrification activity. Deinococcus and Rubrobacterare are radiation resistant strains。From these results we can prove the microbial biodiverisity of thermophilic bacteria in Taiwan. We hope to establish an indigenous thermophilic germplasm of Taiwan.

Selected Publications  

1.) Yang, Y.-L., F.-L. Yang, S.-C. Jao, M.-Y. Chen, S.-S. Tsay, W. Zou and S.-H. Wu. 2006. Structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles Thermus and Meiothermus. Journal of Lipid Research 47: 1823-1832. (SCI)

2.) Chu, S. F., H.-Y. Shu, L.-C. Lin; T.-W. Shao, M.-Y. Chen, S.-S. Tsay and G.-H. Lin. 2006. Characterization of a Rolling-Circle Replication Plasmid from Thermus aquaticus NTU103. Plasmid. (SCI) (accepted)

3.) Yang, F. L., C. P. Lu, C. S. Chen, M. Y. Chen, H. L. Hsiao, Y. Su, S. S. Tsay, W. Zou and S. H. Wu. 2004. Structural determination of the polar glycoglycerolipids from thermophilic bacteria Meiothermus taiwanesis. European Journal of Biochemistry 271: 4545-4551. (SCI)

4.) Liu, T. L., C. S. Chen, F. L. Yang, J. M. Fung, M. Y. Chen, S. S. Tsay, J. Li, W. Zou and S. S. Wu. 2004. Structure of the major glycolipid from Thermus Oshimai NTU-063. Carbohydrate Research 339:2593-2598. (SCI)

5.) Chen, M. Y., S H. Wu, G. H. Lin., C. P. Lu, Y. T. Lin, W. C. Chang and S. S. Tsay. 2004. Rubrobacter taiwanensis sp. nov., a novel thermophilic radiation resistant species isolated from hot spring in Taiwan. International Journal of Systematic and Evolutionary Microbiology 54, 1849-1855. (SCI)

6.) Lee, A. Y. L., S. S. Tsay, M. Y. Chen and S. H. Wu. 2004. Identification of a gene encoding Lon protease from Brevibacillus thermoruber WR-249 and biochemical charaterization of its thermostable recombinant enzyme. European Journal of Biochemistry 271: 834-844. (SCI)

7.) Chen, M. Y., S. S. Tsay, K. Y. Chen, Y. C. Shi, Y. T. Lin and G. H. Lin. 2002. Pseudoxanthomonas taiwanensis sp. nov., a novel thermophilic, N2O-producing species isolated from hot springs. International Journal of Systematic and Evolutionary Microbiology 52: 2155-2161. (SCI)

8.) Chen, M. Y., G. H. Lin, U. T. Lin, and S. S. Tsay. 2002. Meiothermus taiwanensis sp. nov., a novel filamentous, thermophilic species isolated in Taiwan. International Journal of Systematic and Evolutionary Microbiology 52: 1647-1654. (SCI)

9.) Chen, H. P., S. H. Wu, Y. L. Lin, C. M. Chen, and S. S. Tsay. 2001. Cloning, sequencing, heterologous expression, purification and characterzation of adenosylcobalamin-dependent D-ornithine aminomutase from Clostridium sticklandii. Journal of Biological Chemistry 276: 44744-44750. (SCI)

10.) Chen, H. P., C. F. Lin, Y. J. Lee, S. S. Tsay and S.H. Wu, 2000. " Purification and Properties of Ornithine Racemase from Clostridium sticklandii". Journal of Bacteriology. 182(7): 2052-2054. (SCI)

11.) Chen, M. Y., Z. C. Chen., K. Y. Chen and S. S. Tsay. 2000. Fungal flora of hotsprings of Taiwan (1): Wu-Rai. Taiwania 45(2): 207-216

Courses taught 
B01 101B1 普通生物學上
B01 106 B1 普通生物學實驗上
B01 33010 微生物學
B01 33020 微生物學實驗甲
B01 31110 遺傳學
B42 U0320 細菌生理學
B42 U0310 微生物遺傳學
Lab members: 2 graduate students and one undergraduate student
許嘉如(碩一):嗜熱菌Deinococcus sp.之研究
時雨青(碩一):嗜熱菌Pseudoxanthomonas sp.之研究
Copyright © 2004, Institute of Plant Biology, National Taiwan University

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